LB Kan

LB Kan is Lysogeny Broth (LB) containing the antibiotic kanamycin. Because LB is a rich medium for growing bacteria, adding kanamycin provides a means of selecting transformants that have taken up plasmid DNA containing a gene which encodes resistance to kanamycin. Consequently, only bacteria that have been successfully transformed with such a plasmid will survive in LB Kan.

Formula
Dissolve the following in 1L ddH2O:


 * 10g bacto-tryptone
 * 5g bacto-yeast extract
 * 10g NaCl (5g in some recipes)

Adjust to pH 7.0 with 1N NaOH

Autoclave at 121°C for 15-20 minutes

Allow solution to cool to RT

Add 5mL 10mg/mL kanamycin
 * [Kan]final = 50μg/mL

Store at 4°C or RT